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- CD56 (NCAM) Antibodies
Invitrogen
View all (132) CD56 (NCAM) antibodies
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Cite CD56 (NCAM) Monoclonal Antibody (TULY56), Super Bright™ 436, eBioscience™
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CD56 (NCAM) Antibody (62-0566-42) in Flow
Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0038) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714) (left) or CD56 (NCAM) Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
62-0566-42
Applications
Tested Dilution
Publications
Flow Cytometry(Flow)
5 µL (0.25 µg)/test
View 7 publications7 publications
Product Specifications
Species Reactivity
Human,Non-human primate,Rhesus monkey
Published species
Not Applicable
Host/Isotype
Mouse/ IgG1, kappa
Recommended Isotype Control
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
TULY56
Conjugate
Super Bright™ 436 Super Bright™ 436 Super Bright™ 436
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 660-120S
- NovaFluor Yellow 690
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Excitation/Emission Max
413/431 nmView spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2688246
Product Specific Information
Description: This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.
Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB.
The TULY56 monoclonal antibody crossreacts with Rhesus macaque.
Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TULY56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases:antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM
UniProt ID:(Human) P13591
Entrez Gene ID:(Human) 4684, (Rhesus monkey) 693789
Function(s)
virus receptor activityRas guanyl-nucleotide exchange factor activity
Process(es)
MAPK cascadecell adhesionaxon guidancepositive regulation of GTPase activityviral entry into host cellregulation of synaptic plasticityinterferon-gamma-mediated signaling pathway
It has to be done as per old AB suggested Products section.
